KARAKTERISASI AWAL SENYAWA ANTIBIOTIK INHIBITOR -LAKTAMASE TIPE TEM-1 DARI ISOLAT BAKTERI GRAM NEGATIF ICBB 1711

Abstract

Abstract: One strain of rod shaped Gram negative bacteria (ICBB 1171) from Black Water ecosystem, Central Kalimantan has been proven to be able to inhibit the growth of Escherichia coli ATCC 35218 producing TEM-1 b-lactamase enzyme. The objectives of this study were to describe both characters and stability of b-lactamase inhibitor antibiotic of ICBB 1171 isolate to TEM-1 b-lactamase enzyme.  Microbiology and spectrofotometry analyses were applied in this study. The results showed that the character of antibiotic activity of ICBB 1171 isolate was bactericidal. The activity of this antibiotic was relatively unchanged at pH 3.0–9.0, and at temperature of 30-121 ^oC. In contrast, treatment with proteinase-K, decrease antibiotic activity was up to 75%.  This antibiotic is broad spectrum that can inhibit Gram negative and Gram positive bacteria, included Escherichia coli DH5a (pUC 19) encoding ampicillin resistance gene, and two Gram positive bacteria: Bacillus subtilis and Staphylococcus aureus.  The result of spectrofotometry assays proved that antibiotic of ICBB 1171 isolate was stable on crude enzyme of TEM-1 b-lactamase taken from Escherichia coli ATCC 35218.