PROFIL TITER ANTI-TGFβ SERUM DARAH KELINCI YANG DIINDUKSI TGFβ DARI KULTUR COC
Abstract
Abstract: Titer Profile of Blood Serum Rabbit Anti-TGFβ Induced TGFβ of Culture COC. This research was done to determine profile of anti-TGFβ isolated from bovine Cumulus Oocyte Complex (COC) culture. The research was conduced on Biochemistry and Biology Molecular laboratory, Faculty of Mathematics and Natural Science of Brawijaya University, Malang. Anti-TGFβ were produced on Rabbits (Oryctolagus cunniculus) male that induced by TGFβ isolated from bovine COC culture. The spesificity of anti-TGFβ confirmed by Dot Blot and ELISA. The result of Dot Blot showed the violet colour gradation that indicated the concentration anti-TGFβ. Profil of antibody titer anti-TGFβ measured by ELISA that demonstrate or achieve optimum peak at 52 and 88 days after the first immunization based on the value of Optical Density (OD) 405 nm.The conclusion is that TGFβ isolated from bovine COC culture induced the production of antibody to TGFβ and its titer optimum was 1.373.
Abstrak: Profil Titer ANTI-TGFβ Serum Darah Kelinci yang Diinduksi TGFβ dari Kultur COC. Penelitian ini dilakukan untuk menentukan profil antibodi isolat TGFβ dari kultur Cumulus Oocyte Complex (COC) sapi. Penelitian dilakukan di Laboratorium Biokimia dan Biologi Molekuler FMIPA Universitas Brawijaya Malang. Antibodi TGFβ yang diproduksi oleh kelinci (Orytologus cunniculus) jantan diinduksi dari isolat TGFβ kultur sel COC sapi. Uji spesifisitas dilakukan dengan metode Dot Blot dan ELISA. Hasil Dot Blot menunjukkan gradasi warna ungu yang menunjukkan konsentrasi adanya reaksi antigen dan antibodi (TGFβ dan anti- TGFβ). Profil titer anti-TGFβ diukur dengan ELISA menunjukkan mencapai puncak atau optimal pada 52 dan 88 hari setelah imunisasi pertama berdasarkan nilai Optical Density (OD) 405 nm. Kesimpulannya isolasi TGFβ dari kultur COC sapi menginduksi produksi anti- TGFβ dan nilai titer optimum 1,373.