PENGEMBANGAN METODE PENETAPAN KADAR SIKLAMAT BERBASIS KROMATOGRAFI CAIR KINERJA TINGGI GUNA DIIMPLEMENTASIKAN DALAM KAJIAN PAPARAN
Abstract
The aim of this research was to develop a method for determining cyclamate content in foods based on High Performance Liquid Chromatography for exposure assessment of cyclamate. The extraction procedure is conducted by decreasing pH sample until less than 2, and mobile phase is executed by applying the ratio of phosphate buffer-methanol as 75:25, 80:20, 85:15. The results of experiments be obtained colom: ODS RP-18, 5 μm, 250 x 4,6 mm ID; mobile phase: phosphate buffer/KH2PO4 pH 4,6 and metanol in a ratio of 85:15; flow rate: 1 ml/min; injection volume: 20 μl; and detector: UV-Vis 200 nm. The indication of extraction showed by parameter distribution ratio D ≈ KD and the extraction efficiency of 83.04–94.92%. The exposure estimated of cyclamate based concentration analysis is about 28.41 mg/kg bw/day (258.27% ADI), where SNI 01-6993-2004 regulates 2.99 mg/kg bw/day (27.21% ADI).
Tujuan penelitian adalah mengembangkan metode penetapan siklamat berbasis kromatografi cair kinerja tinggi untuk pengukuran konsentrasi siklamat. Prosedur ekstraksi menggunakan modifikasi pH sampel hingga pH lebih kecil 2, dan fase gerak diterapkan perlakuan rasio bufer fosfat–metanol 75 : 25, 80 : 20, dan 85 : 15. Hasil eksperimen diperoleh kolom: ODS RP-18, 5 μm, 250 x 4,6 mm ID; fase gerak: bufer fosfat/KH2PO4 pH 4,6 dan metanol rasio 85 : 15; laju aliran: 1 ml/menit; volume injeksi: 20 μl; dan detektor: UV-Vis 200 nm. Indikasi keberhasilan ekstraksi ditunjukkan dengan parameter rasio distribusi D ≈ KD dan efisiensi ekstraksi 83,04–94,92%. Estimasi paparan berdasar konsentrasi analisis langsung sebesar 28,41 mg/kg bb/hari (258,27% ADI), sedang estimasi paparan berbasis SNI 01-6993-2004 sebesar 2,99 mg/kg bb/hari (27,21% ADI).
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PDFDOI: http://dx.doi.org/10.17977/tk.v33i1.3090
Jurnal Teknologi, Kejuruan, dan Pengajarannya
ISSN 2477-0442 (online)
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